Coding
Part:BBa_K3286207
Designed by: Ben Kuipers Group: iGEM19_Wageningen_UR (2019-10-20)
RpfCch (TMD) sfGFP fusion
This part is similar to part BBa_K3286206, but in this strategy, only the transmembrane domain (TMD) was used to fuse with sfGFP. So again, this part was constructed to test whether part BBa_K3286200 was getting transcribed, translated and transported to the membrane.
Figure 1 and 2 are showing the observed fluorescence by this part. What can be observed is that cells are actually showing a higher intensity of fluorescence at the edges, especially compared to figure 3 which serves as a positive control. Indicating that this part (but also BBa_K3286200) is getting transcribed, translated and transported to the membrane.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 227
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 661
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |